Coding

Part:BBa_I742011:Design

Designed by: Chris French   Group: iGEM07_Edinburgh   (2007-06-27)


ech (feruloyl hydratase/aldolase; vanillin biosynthesis)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 811
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 811
    Illegal NotI site found at 755
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 811
    Illegal NgoMIV site found at 184
    Illegal NgoMIV site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No considerations; this will not be used.


Source

Pseudomonas fluorescens BF13: obtained from GI 4078816 (Barghini et al 2007)

References